基于SPE与SPATT的水体中麻痹性贝类毒素检测方法构建与应用   总被引：1，自引：0，他引：1  

张亚亚  闫国旺  吴海燕  谭志军  张志华  江涛  李玉 《海洋与湖沼》2020,51(2):298-306

为实现麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的实时监控与提前预警,本研究构建了基于固相萃取技术(solid phase extraction,SPE)与固相吸附毒素跟踪技术(solid phase adsorption toxin tracking,SPATT)的水体中PSP检测方法,重点优化了吸附材料及前处理方法,评价了回收率、检出限等指标,并将方法应用于2019年春季秦皇岛山海关海域PSP消长过程的监测中,比较评估了两种方法的监控预警效果。结果表明:SPE方法选用ENVI-Carb 500mg/6mL固相萃取柱,过样体积为50mL,13种PSP组分的平均回收率为82.2%±10.0%、检出限为4.0-20.0ng/L;SPATT方法选用SP207大孔吸附树脂,洗脱时间为静置Id最佳,整体回收率约为9.2%;在实际应用中,结合产毒藻密度及贻贝富集毒素含量的变化,发现SPE方法的检测结果可实时表征海域PSP风险状况,对于贻贝中PSP的预警效果也显著优于SAPTT方法,后者不仅因监控方式相对滞后一个监测周期,且灵敏度及准确性均较差。对于秦皇岛海域,当SPE方法检测结果达到100ng STX eq/L时,该海域贻贝中PSP残留将具有潜在的食用安全风险,跟踪过程表明这一阈值可提前两周预警贻贝富集毒素含量超出我国限量标准(800μg STX eq/kg),这对于强化风险警示并制定防范措施具有积极作用。  相似文献   

守初心耕海探洋七十载担使命披风踏浪砥砺行——中国科学院海洋研究所70年发展纪实   总被引：1，自引：0，他引：1  

王凡  孙松 《海洋与湖沼》2020,51(4):664-672

中国科学院海洋研究所是新中国第一个专门从事海洋科学研究的国立科研机构,她的70年光辉历程是我国海洋科技事业从无到有、从弱到强的发展缩影,是新中国海洋科学调查研究自主创新发展历程的真实写照。本文简要介绍中国科学院海洋研究所的主要发展历程,回顾70年来取得的主要科技成就,并围绕新形势下建设海洋大科学研究中心等改革发展举措,讨论和展望研究所的未来发展。  相似文献   

2013—2019年度海洋科学技术奖计量统计及其科研合作网络分析          下载免费PDF全文

赵晓龙  李保石  王泉斌 《海洋开发与管理》2020,37(11):19-27

文章基于2013—2019年度海洋科学技术奖，运用计量统计、社会网络分析等研究方法，探究海洋科学技术奖获奖成果计量特征、学科分布、获奖机构及其科研合作关系等，直观揭示海洋科学技术奖所构成的科研合作范式、科研合作网络结构等，以期为海洋科研管理、科技创新和学科建设等提供科学参考。研究表明：整体上，海洋科学技术奖获奖成果呈逐年递增趋势，一等奖与二等奖的获奖等级比值呈下降趋势；学科划分上，获奖成果主要隶属于“海洋科学”学科，又以海洋生物学、海洋装备与设备、海洋化学和物理海洋学为主体；获奖机构方面，海洋科学技术奖已奖励包括中国海洋大学和中国科学院海洋研究所等涉海类高校和科研院所在内的各类海洋科技创新主体285家，分布在山东、浙江、广东、上海、北京、江苏等19个省（市、自治区）；科研合作方面，海洋科学技术奖获奖核心机构所构建的科研合作关系紧密，以自然资源部第二海洋研究所的中心度最高，中国海洋大学的中间中心度最高，其控制该科研合作网络中其他机构之间沟通交流的力度最大。  相似文献   

2016与1998年春季北大西洋海表温度异常的差异及成因     

薛文璟  余锦华  陈林 《热带海洋学报》2020,39(3):19-30

利用再分析资料以及混合层海温诊断方程, 研究1997—1998与2015—2016年超级厄尔尼诺次年北大西洋海表温度异常(sea surface temperature anomalies, SSTA)的差异及成因。结果显示, 北大西洋SSTA在1998年春季呈明显正负正三极型式分布, 而在2016年呈弱的负正负型态。诊断热带北大西洋SSTA的影响因素表明, 1998年春季暖SSTA除了之前研究强调的海洋表面向大气的潜热输送异常减少, 以及吸收太阳辐射的增加外, 海洋动力过程即Ekman纬向漂流也起着重要的作用。热力过程与厄尔尼诺峰值后出现的北大西洋涛动(North Atlantic Oscillation, NAO)负位相有关, 其可引起亚速尔高压减弱, 产生西南风异常, 通过风-蒸发-海表温度(sea surface temperature, SST)反馈机制使热带北大西洋蒸发减弱, 海表增暖, 沃克环流下沉支的东移对这一增暖也有贡献。与1997—1998厄尔尼诺事件不同, 2015—2016厄尔尼诺事件没有强迫出负位相NAO, 而是出现弱NAO正位相, 热带北大西洋为弱的东风异常, 使海表发生一定的冷却, 形成2016春季北大西洋SSTA与1998年的明显差异。  相似文献   

Impacts of confining pressure and safety thickness on water and mud inrush in weathered granite     

Jinquan Liu  Weizhong Chen  Zhiping Deng  Taogen Liu 《Marine Georesources & Geotechnology》2020,38(2):144-153

Abstract

Due to the strong disintegration and water erosion of completely weathered granite, water and mud inrush disasters are apt to take place in this zone during underwater tunnel construction. The pore, compactness, seepage path length, fracture geometries and their interconnections for water and mud transfer are strongly influenced by confining pressure and waterproof-resistant slab safety thickness. In order to inspect the influence, a series of experiments based on a self-designed testing system and non-Darcy testing method were performed. The results indicated that the water and mud inrush evolution increased with the increase of confining pressure and decreased with the increase of safety thickness. In particular, the confining pressure mainly influences the initial evolution stage, and a critical safety thickness to prevent water and mud inrush is obtained. Besides, the non-Darcy testing method results shows that the water and mud inrush evolution affects the influence of non-Darcy flow. For example, while the safety thickness was smaller than the critical value, the evolution was large and unstable and its behavior transferred into nonlinear. In this case, the flow changed to non-Darcy flow.  相似文献   

Intra-survey reservoir fluctuations – implications for quantitative 4D seismic analysis     

Veronica Omofoma  Colin MacBeth  Hamed Amini 《Geophysical Prospecting》2019,67(2):282-297

During the time taken for seismic data to be acquired, reservoir pressure may fluctuate as a consequence of field production and operational procedures and fluid fronts may move significantly. These variations prevent accurate quantitative measurement of the reservoir change using 4D seismic data. Modelling studies on the Norne field simulation model using acquisition data from ocean-bottom seismometer and towed streamer systems indicate that the pre-stack intra-survey reservoir fluctuations are important and cannot be neglected. Similarly, the time-lapse seismic image in the post-stack domain does not represent a difference between two states of the reservoir at a unique base and monitor time, but is a mixed version of reality that depends on the sequence and timing of seismic shooting. The outcome is a lack of accuracy in the measurement of reservoir changes using the resulting processed and stacked 4D seismic data. Even for perfect spatial repeatability between surveys, a spatially variant noise floor is still anticipated to remain. For our particular North Sea acquisition data, we find that towed streamer data are more affected than the ocean-bottom seismometer data. We think that this may be typical for towed streamers due to their restricted aperture compared to ocean-bottom seismometer acquisitions, even for a favourable time sequence of shooting and spatial repeatability. Importantly, the pressure signals on the near and far offset stacks commonly used in quantitative 4D seismic inversion are found to be inconsistent due to the acquisition timestamp. Saturation changes at the boundaries of fluid fronts appear to show a similar inconsistency across sub-stacks. We recommend that 4D data are shot in a consistent manner to optimize aerial time coverage, and that additionally, the timestamp of the acquisition should be used to optimize pre-stack quantitative reservoir analysis.  相似文献   

济南东部大辛河渗漏段地下水示踪试验与分析          下载免费PDF全文

丁冠涛  刘玉仙  曹光明  齐欢 《山东国土资源》2018,34(2)

2013年以来,济南市为正确处理好保泉和市民饮用优质地下水的关系,组织实施了地表水转换地下水补源系列工程,旨在补源保泉的前提下开采地下水。大辛河地表水转地下水工程是其中重要的组成部分,为了查明大辛河渗漏补源后地下水的补给方向、径流速度等,在大辛河主要渗漏段开展了地下水示踪试验。结果显示:地下水渗漏补源后沿渗漏段—龙奥大厦—济南东区供水奥体加压站—贤文小区一线大体自南向北径流,视径流速度约45m/d,越往两侧流速越缓慢,表明大辛河地表水转地下水工程主要对东郊水源地进行补给,对市区四大泉群补给较弱。示踪试验得出的结论,对大辛河地表水转地下水工程运行具有重要指导作用,对后期管理部门合理规划补源、开采布局具有重要的借鉴意义,促进济南保泉和供水的有机统一。  相似文献   

Response of hybrid isolation system during a shake table experiment of a full‐scale isolated building     

Keri L. Ryan  Taichiro Okazaki  Camila B. Coria  Eiji Sato  Tomohiro Sasaki 《地震工程与结构动力学》2018,47(11):2214-2232

A full‐scale 5‐story steel moment frame building was subjected to a series of earthquake excitations using the E‐Defense shake table in August, 2011. For one of the test configurations, the building was seismically isolated by a hybrid system of lead‐rubber bearings and low friction roller bearings known as cross‐linear bearings, and was designed for a very rare 100 000‐year return period earthquake at a Central and Eastern US soil site. The building was subject to 15 trials including sinusoidal input, recorded motions and simulated earthquakes, 2D and 3D input, and a range of intensities including some beyond the design basis level. The experimental program was one of the first system‐level full‐scale validations of seismic isolation and the first known full‐scale experiment of a hybrid isolation system incorporating lead‐rubber and low friction bearings. Stable response of the hybrid isolation system was demonstrated at displacement demands up to 550 mm and shear strain in excess of 200%. Torsional amplifications were within the new factor stipulated by the code provisions. Axial force was observed to transfer from the lead‐rubber bearings to the cross‐linear bearings at large displacements, and the force transfer at large displacements exceeded that predicted by basic calculations. The force transfer occurred primarily because of the flexural rigidity of the base diaphragm and the larger vertical stiffness of the cross‐linear bearings relative to the lead‐rubber bearings.  相似文献   

黔北正安地区五峰组—龙马溪组页岩气地质特征及有利区预测     

贺永忠  向坤鹏  安亚运  易成兴  杨忠琴  于宁 《中国地质调查》2020,7(3):21-29

为明确黔北正安地区上奥陶统五峰组至下志留统龙马溪组的页岩气地质特征,充分应用页岩气地质调查、重磁电、二维地震、地质调查井及测试分析等工作方法,开展黔北正安地区1:5万页岩气基础地质调查,对五峰组至龙马溪组富有机质页岩的分布、沉积环境、有机地球化学、岩石矿物、储集性能及含气性等特征进行分析研究,结果表明: 研究区五峰组—龙马溪组富有机质页岩为深水陆棚相沉积,主要分布有安场向斜、斑竹向斜和泥高向斜,厚度14.5~55 m,埋深0~3 200 m; 有机碳(TOC)含量1.0%~4.0%,有机质镜质体反射率(R_o)为1.82%~2.23%,有机质类型以Ⅰ型干酪根为主; 岩石主要由石英、长石和黏土矿物组成,脆性矿物含量高,一般大于50%; 孔隙度为2.03%~3.89%,渗透率为0.35×10^-5~1.86×10^-5μm²,表现为低孔、特低渗的特征; 最高含气量为2.88 m³/t,显示出较好的含气性特征。综合分析和评价圈出3个页岩气聚集有利区,分别为安场区块、斑竹区块和泥高区块,这为研究区页岩气进一步勘探开发指明了方向。  相似文献   

基于深度残差网络的机载LiDAR点云分类     

赵传  郭海涛  卢俊  余东行  张保明 《测绘学报》2020,49(2):202-213

机载LiDAR点云的分类是利用其进行城市场景三维重建的关键步骤之一。为充分利用现有的图像领域性能较好的深度学习网络模型,提高点云分类精度,并降低训练时间和对训练样本数量的要求,本文提出一种基于深度残差网络的机载LiDAR点云分类方法。首先提取归一化高程、表面变化率、强度和归一化植被指数4种具有较高区分度的点云低层次特征;然后通过设置不同的邻域大小和视角,利用所提出的点云特征图生成策略,得到多尺度和多视角点云特征图;再将点云特征图输入到预训练的深度残差网络,提取多尺度和多视角深层次特征;最后构建并训练神经网络分类器,利用训练的模型对待分类点云进行预测,经后处理得到分类结果。利用ISPRS三维语义标记竞赛的公开标准数据集进行试验,结果表明,本文方法可有效区分建筑物、地面、车辆等8类地物,分类结果的总体精度为87.1%,可为城市场景三维重建提供可靠的信息。  相似文献